Studies on Proteolysis in Stored Muscle

Measurement of muscle proteolysis and the impact on muscle wasting.

In general terms, muscle mass begins to decrease after the age of 30 years and continues to decrease at varying rates throughout the rest of life (see Fig. 1; Forbes & Reina, 1970). As human subjects approach 75-80 years of age they may have lost as much as 40 % of the peak muscle mass which places them very near the minimum point of critical muscle mass necessary to maintain both ambulatory an...

Ubiquitin-proteasome-dependent proteolysis in skeletal muscle.

The ubiquitin-proteasome proteolytic pathway has recently been reported to be of major importance in the breakdown of skeletal muscle proteins. The first step in this pathway is the covalent attachment of polyubiquitin chains to the targeted protein. Polyubiquitylated proteins are then recognized and degraded by the 26S proteasome complex. In this review, we critically analyse recent findings i...

Effect of experimental hyperthyroidism on skeletal-muscle proteolysis.

It is not clear whether the muscle wasting commonly observed in hyperthyroidism is due to alteration in the rate of protein synthesis or degradation. The effect of experimental hyperthyroidism on skeletal-muscle proteolysis in the rat was studied by measuring alanine and tyrosine release from isolated skeletal muscles in vitro and 3-methyl-histidine excretion in vivo. Alanine release from the i...

Spectrophotometric Studies on Intact Muscle

The concentrations of the components of the respiratory chain were determined in a variety of intact skeletal muscles by a method of spectrophotometric observation of the transmitted light. In the case of the toad sartorius, these measurements were checked against isolated mitochondrial suspensions prepared from toad skeletal muscles. The relative concentrations of the respiratory components we...

Studies on nebulin in amphioxus striated muscle